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Gimpel, J.A., Vinyard, D.J., Ananyev, G.M., Mayfield, S.P., Dismukes, G.C. (2014) Engineered Photosystem II reaction centers optimize photochemistry vs. photoprotection at different solar intensities. Journal of the American Chemical Society. In press.

Rasala BA, Chao S-S, Pier M, Barrera DJ, Mayfield SP (2014) Enhanced Genetic Tools for Engineering Multigene Traits into Green Algae. PLoS ONE 9(4): e94028.

Rasala BA, Mayfield SP. (2014) Photosynthetic Biomanufacturing In Green Algae; Production Of Recombinant Proteins For Industrial, Nutritional, And Medical Uses. Photosynthetic Research. In press.

Barrera, D.J., Gimpel J.A., Mayfield, S.P. (2014) Rapid Screening for the Robust Expression of Recombinant Proteins in Algal Plastids. In Chloroplast Biotechnology - Methods and Protocols. P. Maliga, editor. Springer-Verlag, New York.

Specht EA, Mayfield SP. (2014) Algae-based oral recombinant vaccines. Front. Microbiol. 5:60.

Specht EA,Nour-Eldin HH, Hoang KTD, and Mayfield, SP. (2014) An improved ARS2-derived nuclear reporter enhances the efficiency and ease of genetic engineering in Chlamydomonas. Biotechnology Journal.


Barrera, D. J., Mayfield, S. P. (2013) High-value Recombinant Protein Production in Microalgae. Handb. Microalgal Cult. (Emeritus, A. R. P. D. & Ph.D, Q. H.) 532–544 (John Wiley & Sons, Ltd.).

Gimpel, J.A., Mayfield S.P. (2013) Analysis of heterologous regulatory and coding regions in algal chloroplasts. Applied microbiology and biotechnology. 97:4499-4510.

Gimpel JA, Specht EA, Georgianna DR, Mayfield SP. (2013) Advances in microalgae engineering and synthetic biology applications for biofuel production. Current Opinion in Chemical Biology, 17:489–495.

Specht EA, Mayfield SP. (2013) Synthetic oligonucleotide libraries reveal novel regulatory elements in Chlamydomonas chloroplast mRNAs. American Chemical Society Synthetic Biology, 2(1): 34-46.

Rasala BA, Barrera DJ, Ng J, Plucinak TM, Rosenberg JN, Weeks DP, Oyler GA, Peterson TC, Haerizadeh F, Mayfield SP. (2013) Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii. The Plant Journal, 74(4):545-56.

Rasala BA, Gimpel J, Tran M, Hannon MJ, Miyake-Stone SJ, Specht EA, Mayfield SP. (2013) Genetic engineering to improve algal biofuels production. In Algae for Biofuels and Energy (Michael A. Borowitzka, ed), Springer, New York, pp. 99-114.

Tran M, Henry RE, Siefker D, Van C, Newkirk G, Kim J, Bui J, Mayfield SP. (2013) Production of anti-cancer immunotoxins in algae: ribosome inactivating proteins as fusion partners. Biotechnology and bioengineering, 110(11):2826-2835.

Tran, M., Van, C., Barrera, D.J., Pettersson, P.L., Peinado, C.D., Bui, J., Mayfield, S.P. (2013) Production of unique immunotoxin cancer therapeutics in algal chloroplasts. Proc. Natl. Acad. Sci. U. S. A. 110, E15–22.

Vinyard, D.J., J. Gimpel, G.M. Ananyev, M.A. Cornejo, S.S. Golden, S.P. Mayfield, & G.C. Dismukes. (2013) Natural variants of Photosystem II subunit D1 tune photochemical fitness to solar intensity. Journal of Biological Chemistry. 288:5451-5462.


Blatti, J. L., Beld, J., Behnke, C. A., Mendez, M., Mayfield, S. P., & Burkart, M. D. (2012). Manipulating Fatty Acid Biosynthesis in Microalgae for Biofuel through Protein-Protein Interactions. PLOS ONE, 7(9), e42949.

Georgianna DR, Mayfield SP. Exploiting diversity and synthetic biology for the production of algal biofuels. Nature. 2012 Aug 16;488(7411):329-35.

Georgianna, D. R., Hannon, M. J., Marcuschi, M., Wu, S., Botsch, K., Lewis, A. J., ... & Mayfield, S. P. (2012). Production of recombinant enzymes in the marine alga Dunaliella tertiolecta. Algal Research.

Gimpel, J. A., & Mayfield, S. P. (2012). Analysis of heterologous regulatory and coding regions in algal chloroplasts. Applied Microbiology and Biotechnology, 1-12.

Gregory, J.A., Li, F., Tomosada, L., Cox, C.,Aaron Topol, A., Vinetz, J.M., Stephen Mayfield, S.P. (2012) Algae-produced Pfs25 elicits antibodies that inhibit malaria transmission. PLoSOne 7:1-10

Jones CS, Luong T, Hannon M, Tran M, Gregory JA, Shen Z, Briggs SP, Mayfield SP.Heterologous expression of the C-terminal antigenic domain of the malaria vaccine candidate Pfs48/45 in the green algae Chlamydomonas reinhardtii. Appl Microbiol Biotechnol. 2012 May 18.

Jones CS, Mayfield SP. Algae biofuels: versatility for the future of bioenergy. Curr Opin Biotechnol. 2012 Jun;23(3):346-51.

Limtiaco JK, Pomeroy RS, Burkart M, Mayfield S. (2012) Educating and Developing Workers for the Green Economy. Biofuels. 3(2):199-121

Rasala, B., Lee, P., Shen, X., Briggs, S., Mendez, M., Mayfield, S.P. (2012) Robust expression and secretion of xylanase1 in Chlamydomonas reinhardtii by fusion to a selection gene and processing with the FMDV 2A peptide. PLoSOne, 7(8):e43349

Tran, M., Van, C., Barrera, D. J., Pettersson, P. L., Peinado, C. D., Bui, J., & Mayfield, S. P. (2012). Production of unique immunotoxin cancer therapeutics in algal chloroplasts. Proceedings of the National Academy of Sciences.



Coragliotti AT, Beligni MV, Franklin SE, Mayfield SP. Molecular Factors Affecting the Accumulation of Recombinant Proteins in the Chlamydomonas reinhardtii Chloroplast. Mol Biotechnol.2011 May;48(1):60-75.

Rasala, B., Mayfield, S.P. The microalga Chlamydomonas reinhardtii as a platform for the production of human protein therapeutics. BioEngineered Bugs. 2011 Jan-Feb;2(1):50-4.

Rasala BA, Muto M, Sullivan J, Mayfield SP. Improved heterologous protein expression in the chloroplast of Chlamydomonas reinhardtii through promoter and 5' untranslated region optimization. Plant Biotechnol J. 2011 Aug;9(6):674-83.

Yu WL, Ansari W, Schoepp NG, Hannon MJ, Mayfield SP, Burkart MD. Modifications of the metabolic pathways of lipid and triacylglycerol production in microalgae. Microb Cell Fact. 2011 Nov 2;10:91. Review.


Hannon. M., Gimpel, J., Tran, M., Rasala, B. Mayfield, S.P. (2010) Biofuels from algae: challenges and potential. Biofuels 1:91-103.

Rasala, B., Muto, M., Lee, P., Jager, M., Cardoso, R., Behnke, C., Kirk, P., Hokanson, C., Crea, R., Mendez, M., Mayfield, S.P. (2010) Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii. Plant Biotech J. 8:1-15.

Rasala BA, Gimpel JA, Tran M, Hannon MJ, Miyake-Stoner SJ, Specht EA, Mayfield SP. (2010) Genetic Engineering to improve algal biofuel production. In press

Specht E, Miyake-Stoner S, Mayfield S. (2010) Micro-algae come of age as a platform for recombinant protein production. Biotechnol Lett. 32:1373-83.

Tran, M., Mayfield, S.P. (2010) Expression of Full Length Monoclonal Antibodies (mAb) in Algal Chloroplast Antibody Engineering, R. Kontermann and S. Du¨bel (eds.), Springer-Verlag Berlin Heidelberg.




Merchant et al. (2007) The Chlamydomonas genome reveals the evolution of key animal and plant functions. Science. 318; 245-50.

Mayfield, S.P., Manuell, A.L., Chen, S., Wu, J., Tran, M., Siefker, D., Muto, M., Marin-Navarro, J. (2007) Chlamydomonas reinhardtii chloroplasts as protein factories. Curr. Opin Biotech. 18:126-33.

Marin-Navarro J, Manuell AL, Wu J, Mayfield SP. Chloroplast translation regulation. Photosynthesis Research. 2007 July; ePub ahead of print.

Manuell AL, Quispe J, Mayfield SP. Structure of the Chloroplast Ribosome: novel domains for translation regulation. PLoS Biology. 2007 August;5(8):e209.

Manuell AL, Beligni MV, Elder JH, Siefker DT, Tran M, Weber A, McDonald TL, Mayfield SP. Robust expression of a bioaactive mammalian protein in Chlamydomonas chloroplast. Plant Biotechnology Journal. 2007 May;5(3):402.

Mayfield SP, Manuell AL, Chen S, Wu J, Tran M, Siefker D, Muto M, Marin-Navarro J. Chlamydomonas reinhardtii chloroplasts as protein factories. Current Opinion in Biotechnology. 2007 April;18(2): 126.



Barnes D, Franklin S, Schultz J, Henry R, Brown E, Coragliotti A, Mayfield SP. Contribution of 5'- and 3'-untranslated regions of plastid mRNAs to the expression of Chlamydomonas reinhardtii chloroplast genes. Molecular Genetics and Genomics. 2005 December;274(6):625-36.

Manuell AL, Yamaguchi K, Haynes PA, Milligan RA, Mayfield SP. Composition and structure of the 80S ribosome from the green alga Chlamydomonas reinhardtii: 80S ribosomes are conserved in plants and animals. Journal of Molecular Biology. 2005 August;351(2):266-79.

Somanchi A, Barnes D, Mayfield SP. A nuclear gene of Chlamydomonas reinhardtii, Tba1, encodes a putative oxidoreductase required for translation of the chloroplast psbA mRNA. The Plant Journal. 2005 May;42(3):341-52.

Mayfield SP, Franklin SE. Expression of human antibodies in eukaryotic micro-algae. Vaccine. 2005 March;23(15):1828-32.

Franklin SE, Mayfield SP. Recent developments in the production of human therapeutic proteins in eukaryotic algae. Expert Opinion on Biological Therapy. 2005 February;5(2):225-35.


Beligni MV, Yamaguchi K, Mayfield SP. The translational apparatus of Chlamydomonas reinhardtii chloroplast. Photosynthesis Research. 2004 December;82(3):315-25.

Beligni MV, Yamaguchi K, Mayfield SP. Chloroplast elongation factor ts pro-protein is an evolutionarily conserved fusion with the S1 domain-containing plastid-specific ribosomal protein-7. The Plant Cell. 2004 December;16(12):3357-69.

Manuell A, Beligni MV, Yamaguchi K, Mayfield SP. Regulation of chloroplast translation: interactions of RNA elements, RNA-binding proteins and the plastid ribosome. Biochemical Society Transactions. 2004 August;32(Pt 4):601-5.

Barnes D, Cohen A, Bruick RK, Kantardjieff K, Fowler S, Efuet E, Mayfield SP. Identification and characterization of a novel RNA binding protein that associates with the 5'-untranslated region of the chloroplast psbA mRNA. Biochemistry. 2004 July;43(26):8541-50.

Franklin SE, Mayfield SP. Prospects for molecular farming in the green alga Chlamydomonas. Current Opinion in Plant Biology. 2004 April;7(2):159-65.

Mayfield SP, Schultz J. Development of a luciferase reporter gene, luxCt, for Chlamydomonas reinhardtii chloroplast. The Plant Journal. 2004 February;37(3):449-58.



Yamaguchi K, Prieto S, Beligni MV, Haynes PA, McDonald WH, Yates JR 3rd, Mayfield SP. Proteomic characterization of the small subunit of Chlamydomonas reinhardii chloroplast ribosome: identification of a novel S1 domain-containing protein and unusually large orthologs of bacterial S2, S3, and S5. The Plant Cell. 2002 November;14(11):2957-74.

Kim J, Mayfield SP. The active site of the thioredoxin-like domain of chloroplast protein disulfide isomerase, RB60, catalyzes the redox-regulated binding of chloroplast poly(A)-binding protein, RB47, to the 5' untranslated region of psbA mRNA. Plant and Cell Physiology. 2002 October;43(10):1238-43.

Franklin SE, Ngo B, Efuet E, Mayfield SP. Development of a GFP reporter gene for Chlamydomonas reinhardtii chloroplast. The Plant Journal. 2002 June;30(6):733-44.




Somanchi A, Mayfield SP. Nuclear-chloroplast signalling. Current Opinion in Plant Biology. 1999 October;2(5):404-9.

Bruick RK, Mayfield SP. Light-activated translation of chloroplast mRNAs. Trends in Plant Science. 1999 May;4(5):190-5.

Bruick RK, Mayfield SP. Processing of the psbA 5' untranslated region in Chlamydomonas reinhardtii depends upon factors mediating ribosome association. The Journal of Cell Biology. 1998 November;143(5):1145-53.

Yohn CB, Cohen A, Rosch C, Kuchka MR, Mayfield SP. Translation of the chloroplast psbA mRNA requires the nuclear-encoded poly(A)-binding protein, RB47. The Journal of Cell Biology. 1998 July;142(2):435-42.

Kim J, Mayfield SP. Protein disulfide isomerase as a regulator of chloroplast translational activation. Science. 1997 December;278(5345):1954-7.

Cohen A, Mayfield SP. Translational regulation of gene expression in plants. Current Opinion in Biotechnology. 1997 April;8(2):189-94.

Cravatt BF, Giang DK, Mayfield SP, Boger DL, Lerner RA, Gilula NB. Molecular characterization of an enzyme that degrades neuromodulatory fatty-acid amides. Nature. 1996 November;384(6604):83-7.

Yohn CB, Cohen A, Danon A, Mayfield SP. Altered mRNA binding activity and decreased translational initiation in a nuclear mutant lacking translation of the chloropalst psbA mRNA. Molecular and Cellular Biology. 1996 July;16(7):3560-6.

Yohn CB, Cohen A, Danon A, Mayfield SP. A poly(A) binding protein functions in the chloroplast as a message-specific translation factor. Proceedings of the National Academy of Scienced of the United States of America. 1998 March;95(5):2238-43.

Mayfield SP, Yohn CB, Cohen A, Danon A. Regulation of chloroplast gene expression. Annual Review of Plant Physiology and Plant Molecular Biology. 1995 June;46:147-166.

Mayfield SP. Chloroplast gene regulation: interaction of the nuclear and chloroplast genomes in the expression of photosynthetic proteins. Current Opinion in Cell Biology. 1990 June;2(3):509-13.

Mayfield SP, Kindle KL. Stable nuclear transformation of Chlamydomonas reinhardtii by using a C. reinhardtii gene as the selectable marker. Proceedings of the National Academy of Sciences of the United States of America. 1990 March;87(6):2087-91.

Danon A, Mayfield SP. Light-regulated translation of chloroplast messenger RNAs through redox potential. Science. 1994 December;266(5191):1717-9.

Mayfield SP, Cohen A, Danon A, Yohn CB. Translation of the psbA mRNA of Chlamydomonas reinhardtii requires a structured RNA element contained within the 5' untranslated region. The Journal of Cell Biology. 1994 December;127(6 Pt 1):1537-45.

Danon A, Mayfield SP. ADP-dependent phosphorylation regulates RNA-binding in vitro: implications in light-modulated translation. The EMBO Journal. 1994 May;13(9):2227-35.

Danon A, Mayfield SP. Light regulated translational activators: identification of chloroplast gene specific mRNA binding proteins. The EMBO Journal. 1991 December;10(13):3993-4001.